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KMID : 1005220160310010081
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Journal of Embryo Transfer
2016 Volume.31 No. 1 p.81 ~ p.88
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Study on Distribution of Oct4 Expression and Change of Apoptosis in Nuclear Transfer Blastocyst using Oct4-Transfected Mesenchymal Stem Cells
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Lee Won-Jae
Lee Jeong-Hyeon
Rho Gyu-Jin
Lee Sung-Lim
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Abstract
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There are various factors i.e. donor cell type, culture system as well as technical procedures which influence the pre-implantation embryonic development; however, may attempts have been made and still it is under investigation to improve the cloning efficiency using somatic cell nuclear transfer technique. It is has been investigated that stem cells like mesenchymal stem cell are able to more efficiently reprogram and reactivate the expression of early embryonic genes to promote nuclear transfer efficiency. In addition, Oct4 expression plays a pivotal role in early embryo development. In the present study, we investigated distribution of Oct4 expression and changes of apoptosis and total cell number in nuclear transfer blastocyst after using Oct4 transfected bone marrow stem cell as donor cells. Although Oct4-RFP expression was observed across blastocyst, more concentrated intensity was shown at hatched region in blastocyst on day 7. Reduction of apoptotic bodies was revealed in Oct4 transfected blastocyst by TUNEL staining, however, there was no significant difference in total cell number between Oct4 transfected and non-transfected nuclear transfer embryos. In conclusion, Oct4 transfected donor cells exhibited higher expression in hatching sight in day 7 blastocyst and were able to prevent apoptosis compared to non-transfected donor cells.
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KEYWORD
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mesenchymal stem cells , bone marrow , porcine , Oct4 , apoptosis
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